FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_F07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_F07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8581103
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG38800.020.7402275
GTATTAG72400.018.5450881
GTATAGA76400.017.5741421
CTAATAC77100.017.4895383
ACTATGC71750.017.3791628
TATACAG96300.016.9386395
GTATATA177350.016.8601651
CTATGCA60100.015.6820859
TATACTG108800.015.5922665
TAGTCAG41050.015.0120749
TAATACT128450.014.6743544
ACTGTGC35900.014.5398648
TATACGG52900.014.5268845
GTATATG103950.014.3128171
TATTAGG82200.014.28805352
TACACCG29950.014.281625
TACTATG80400.014.0666557
CCTAATA81400.013.9831542
GGTAGTC52000.013.9418027
GTCTAGG34200.013.5815581
Produced by FastQC (version 0.11.5)