FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_F05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_F05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235513
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG17400.022.9432071
TAGGTAG10100.020.098435
ACTATGC17800.019.142718
TATACGG11300.018.6056615
GTATTAT32950.018.2836691
GTATAGA18050.018.0957261
GTGTAAG10900.017.9795131
CTAATAC18750.017.3995023
TATTAGG18400.017.3364582
TCTATAC18400.017.3364583
CCGTGCG2550.001592130717.0583349
GTACTAG11500.017.0414521
CCTAATA18350.016.9886152
TAATACT28700.016.1667844
GGTAGTC10000.015.9495427
TTATACT23700.015.9067164
GTATAAG18850.015.7874931
TATACTG22500.015.4662245
CTATACT17550.015.2844614
CTCTAGG6251.0360964E-815.0974641
Produced by FastQC (version 0.11.5)