FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_F02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_F02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744903
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG14150.022.0544931
TCGGTCG3757.772542E-921.266619
TAGGTAG8000.020.8430945
GGTAGTC8400.018.98757
TATACAG20250.017.5426585
GTCTTAG8500.017.0763931
GTATAGA15750.017.0492881
TATACGG10000.016.6744775
ACCGCGC2650.002059023116.4145778
GTATAGG11150.016.2723481
TATTAGG15650.015.7512062
AGACTGC5705.5588316E-815.2626796
ATACGGT10100.015.0737846
TAGTCAG8751.8189894E-1214.9142459
TAGACAG10300.014.7810895
GTATATA33050.014.7125661
GTATTAA17000.014.5149341
TACGGTG9001.8189894E-1214.4995447
ATAATAC28050.014.4741133
CTAATAC15050.014.4517873
Produced by FastQC (version 0.11.5)