FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_E12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_E12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035412
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGC3550.030.631038
GGACCGC1204.0602993E-430.2055976
TGGACCG2003.2571006E-728.9973725
CCGCGCG1050.007708350727.6192119
ACTATGC6200.026.8927278
GTCTTAG4650.024.968091
CTAATAC6950.023.9906313
TAGGACA3052.0367224E-823.768344
GTATTAG5950.020.7324331
CTGTGCG1750.003658689620.714419
TCCTAGG1750.003659713520.713412
CAGCGCA2908.249526E-620.0001189
CCCCTAA2959.4907045E-619.6782421
CCTGTCG3051.2760116E-519.0146718
CTTAGAC5056.202754E-1018.6616763
CTCCTAG1950.006806229718.6060311
AGGAGCG2000.00792039718.1233585
TACTATG6401.8189894E-1218.1233587
CTAGGAC2400.001060389718.1233583
CTATGCA6052.0008883E-1117.9753159
Produced by FastQC (version 0.11.5)