FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_E11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_E11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3600883
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG28300.019.7445961
GTATAGA33750.016.7712251
TAGGTAG16350.016.4063385
TATACGG22900.016.1459055
TAGACCG15600.015.8009065
GTATATA73150.015.4758421
CTAATAC33700.015.2741173
CCGCGCA6209.469659E-915.2014639
ACTGTGC16500.014.9390398
TATACTG46950.014.6695135
TAGGACG8901.8189894E-1214.6625734
ACTATGC30800.013.8876578
CTAGGAC11000.013.8405813
TATTATA89500.013.7706222
ATACTAT55350.013.7530616
GTATAGG22700.013.7463251
CTACACC23300.013.6906614
TCTACAC25450.013.6735463
TATACAG37850.013.5994135
TCCTAGA17800.013.4406922
Produced by FastQC (version 0.11.5)