FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_E10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_E10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105328
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAC100.0070822667144.997622
AGCCCTA100.0070822667144.997627
CTGTTAG350.003524511862.2897261
TAAGCCG502.00212E-457.9990465
TTAGGGT400.006036313354.3741074
CCTGTAC400.006036313354.3741073
TCCGTTT400.006036313354.3741078
CTATATA450.00961600848.3325424
ACCTGTA450.00961600848.3325422
TGCGCTT450.00961600848.3325427
GTTCCCA450.00961600848.3325429
CTAATAC752.5351792E-548.3325423
TATAACT657.3071924E-444.6146554
CTTTGCA1001.3831467E-436.2494059
CAGTACC1051.8426824E-434.5232434
GGTATAA850.002731131234.117092
TCAAAGC850.002731131234.117093
CCAGTAC900.003613910632.2216953
CCAACAT950.004708692430.5258163
TCTACAA1000.006050624428.9995232
Produced by FastQC (version 0.11.5)