FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_E09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_E09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379155
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG2257.930794E-1032.2219735
CCTAGGG1652.38744E-630.7573382
GTACTAG5950.028.0565241
TACACCG3403.6379788E-1227.7203735
GTATTAG8500.027.3246151
GTCTAGG5100.025.6168271
CTAGGAC2951.4289981E-824.576083
GGGCCCG6450.023.6044677
CCCTAGG1851.8312529E-423.513332
TATACTG11800.020.8896685
GGGGCCC6950.019.819996
TCCTAGG2205.90523E-419.7725732
GCCCGGC6600.019.7725739
TAGTCCG6000.019.3331839
ATAGTCC7900.019.2720038
TCCGGGG8400.018.9879473
GGCCCGG7650.018.9541028
ACAGGTC5407.4578566E-1118.796158
TATACCG4654.5019988E-918.7095325
GTCCCAG5509.640644E-1118.4751661
Produced by FastQC (version 0.11.5)