FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_E08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_E08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4234826
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG18250.025.820575
GTATTAG36100.024.3251341
CTAATAC40000.023.1987923
TACACCG16550.022.3402255
ACTGTGC20200.022.2513148
TAGACCG24850.021.588415
GTATAGA40100.021.3557431
TATACCG23650.021.1511155
TATTAGG38100.020.7406332
CCTAATA39550.019.9802282
ACTATGC35050.019.6494738
TATACAG45800.019.3112185
TAGTCAG21050.019.2870869
GTCCTAG12850.019.2022821
GTCCTAC11250.018.7077921
ATACCGT34250.018.2034286
TATACTG51800.018.194035
TAATACT64600.017.7312744
CTATGCA29700.017.5754629
GGTAGTC25200.017.5486877
Produced by FastQC (version 0.11.5)