FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_E07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_E07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3890547
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG17650.022.1809625
GTATTAG31300.018.0844781
CTAATAC34900.018.0727883
TATTAGG37750.017.4767782
GTATATA84000.017.1057321
TATACCG21650.016.7433915
GGACCGC5302.2737368E-816.4148436
TAATACT57350.015.9282654
TATACTG50550.015.77621655
GTATAGA36600.015.2674091
TAGTCAG20500.015.2070849
TATACAG44600.015.1174815
TATACGG24050.015.0725355
TACTATG38550.015.0451647
TCTATAC33450.014.9549273
TATATAC92500.014.8916643
ACCGTCG16600.014.849178
GTCCTAC10100.014.3702521
GGTAGTC21200.014.3629877
CCTAATA37900.014.34693052
Produced by FastQC (version 0.11.5)