FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_E06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_E06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379445
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20420.14803054851770095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG10100.024.423931
GTATAAG11650.022.4199471
GTATAGA10200.021.3392471
GGAGCGC2104.3181074E-420.712276
TAGGTAG6250.019.7180825
TAGTCAG5153.8198777E-1119.7075359
CCCTACG1850.005050669419.592697
CTTACAC6300.019.56233
TATAAGA14500.019.499522
TATACGG7100.019.3995235
TATTAGG10750.018.208852
GTCTTAG5601.2551027E-1018.138361
CCGTGCC2400.001060421918.1238949
CTAGACT3652.9884031E-617.8756224
ACTAGGC3352.860993E-517.3123769
TAAGAGA15700.016.623194
GTATAGG7950.016.4271951
ACCCCGT3103.107073E-416.3693758
AGGACGG3655.967431E-515.8888655
GAGACAG10500.015.8794087
Produced by FastQC (version 0.11.5)