FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_E05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_E05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490934
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC2755.2141568E-621.0906016
ACCGCGC1750.003659869820.7139848
GCACGCC3101.4677496E-518.7094069
TCCTAGG3302.5128249E-517.576092
TACACCG4608.09905E-817.3367025
AAGACCG6455.2750693E-1116.8602185
CATATAC15100.016.8043923
GCCCGGC3905.616388E-616.7305269
CTAGGAC3504.1642998E-516.5711883
GTATAAG10100.016.5285071
GTATTAT22550.016.4153751
TAGGTAG5904.9076334E-915.9743435
TATACAG15000.015.9497675
TAGGACG2750.002636134615.8179514
GTATTAG9750.015.6329831
TACGGGT3254.4497507E-415.6151564
GTATAGT10750.015.5291091
CCGTACA5654.9862138E-815.3980069
CTCTTAG7603.8198777E-1115.2803581
AGACGGC4301.41845685E-515.1741986
Produced by FastQC (version 0.11.5)