FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_E04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_E04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7838359
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG54850.017.0689851
GTATTAG53300.017.02071
GTATAGA53550.015.5859391
TATACCG34550.015.3177665
GTATTAT100200.015.2830261
TATACGG40000.014.4994195
GTATATA106550.014.2359821
TAATACT89650.014.0708254
GTGTTAG47600.013.8748741
GTATAGG42650.013.7835341
TATAAGA71700.013.6504292
TAGACAG43800.013.57251555
TCTATAC56550.013.3327993
CTAATAC58250.013.3170633
ACCGCGC12000.013.291058
TATATAC105750.013.231153
TAGGTAG29050.013.22666555
CTAGGAC20950.013.1498313
TATAATA116250.013.034222
ACTATGC55150.013.0139028
Produced by FastQC (version 0.11.5)