FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_E03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_E03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30472597
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG228900.021.7824331
GTATTAG211950.019.4838281
GTATAGA222000.019.0594791
TAGGTAG113050.018.3407365
TATAAGA293800.016.9770222
TAATACT347000.016.6722684
CTAATAC234100.016.5371693
TATTAGG207750.015.8081852
ATAAGAG261400.015.6143573
GTATTAT382750.015.3590821
GTATATA385650.014.8295611
TATACTG296650.014.6387425
TATACAG235400.014.6287925
CCTAATA215900.014.6070152
GTACTAG145800.014.3858581
TTATACT300050.014.2070854
TATACCG138550.014.0232775
ACTATGC227850.013.9998778
CTATGCA182800.013.8414619
TACACCG103200.013.5581035
Produced by FastQC (version 0.11.5)