FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_E01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_E01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3466066
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54900.15839282921906278No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG28050.022.2507171
CTAGGAC10650.021.782863
TATACAG34600.020.3240475
CTAATAC31000.018.7086683
TATTAGG27400.018.2565772
TAGGTAG15650.018.0661165
CCTAATA29400.018.0009882
ACTATGC28800.017.87238
GTATAGA28700.017.7008671
TATACTG39000.016.543985
TAGGACC12650.016.0465644
TATACCG18950.015.6851685
TATAGGG17700.015.5643562
CATATAC36250.015.3991673
CTAGACA20300.014.999194
TAGTCAG16050.014.9065979
TATACGG18000.014.9019735
CTATGCA24850.014.8793319
TAATACT47300.014.8670624
GTCTAGG15650.014.8393211
Produced by FastQC (version 0.11.5)