FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_D11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_D11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4565546
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG35300.018.9137821
GTATATA99700.017.3239561
TAGGTAG21400.016.261295
GTATAGA41000.015.9302991
CTAATAC40150.015.348323
TATACAG53250.015.112315
TATACGG30900.015.0158185
GTATAAG38200.014.4382811
ACTATGC37350.013.005048
ATACTAT70850.012.9954516
TACTATG43600.012.9698697
TATATAC108100.012.8095883
CTATGCA31750.012.7873729
TATTAGG46850.012.6891272
CATATAC58850.012.565543
ACGGTGT30150.012.2633858
CCTAATA49600.012.1317632
CTAGGAC13150.012.12898353
TACGGTG26900.012.1279597
TACACCG16500.011.8633495
Produced by FastQC (version 0.11.5)