FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_D10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_D10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645091
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8730.13532974417562793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCG3050.033.276759
GGACCGT1402.7462698E-531.0696186
CGGGACA950.004727411530.524544
TATAGTG3251.8189894E-1228.9983085
GTATTAG4300.025.3225941
TAGGACC2601.1440716E-725.094694
GTACACT2051.2899231E-524.7546544
TGAGCGC1550.001808409823.3857335
TCTATAC4154.3655746E-1122.709523
GGAGTGT3203.3829565E-822.6549326
ACTCCGC1952.6143793E-422.3063938
GACCGTA1650.002600747921.9684187
GGGTTAT2704.392563E-621.5085721
GAGTGTC1700.003092663421.3222897
TCGGGCT1700.003092663421.3222894
GTATATG4451.10958354E-1021.2064861
GTCTAAG2404.317643E-521.17251
AGTACTC2404.3617572E-521.14465
GTATAAG4501.2914825E-1020.970861
TAGGCCT2455.1126874E-520.713084
Produced by FastQC (version 0.11.5)