FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_D08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_D08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7215988
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC10550.020.6149048
GTATTAG48000.018.2951
CTAGACT21900.017.8757884
TATACCG29050.017.7185345
CTAATAC52650.017.4872253
GACCGCG11850.017.1299157
TAGACCG30600.016.8210265
CTATACT45700.016.8153254
GTATAGG35600.016.51291
TAGGTAG24000.016.3116575
GTATAGA50450.016.2556951
TAATACT83750.015.9275354
GTACTAG30450.015.7305871
GTCCTAG16200.015.6798271
TACACCG21350.015.280245
AGACCGC21500.015.1736346
TATACAG48800.015.1529055
TCTATAC46500.015.1228733
TATACTG67350.014.7468255
CCCCTAG9900.014.6616571
Produced by FastQC (version 0.11.5)