FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_D07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_D07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4316983
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG21250.027.2925625
GTATTAG35150.024.9822371
ACCGTGC9600.022.6546978
GTATAGA43400.021.2366121
CTAATAC41350.019.8114453
TACACCG16850.019.7911595
TATACAG50050.018.8300975
TAGTCAG22350.018.8143359
GTCCTAG12150.018.5164051
GGTAGTC25850.018.5095847
TCTATAC40000.018.4864483
TATACCG23200.018.4364515
TATACGG26000.018.40285
CTATGCA34050.018.3113259
TATACTG52500.018.2275335
GTATATA91500.018.0836051
ACTATGC40100.017.5362038
TAGACCG25050.017.364285
TTAGGTA37800.016.8773464
TATTAGG42450.016.7365782
Produced by FastQC (version 0.11.5)