FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_D06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_D06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557122
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15630.10037749129483753No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAGG1658.372518E-526.3862691
ACCGCGC2701.6502418E-724.1664438
GTATAGA13300.023.459971
GTATTAG12950.022.9733731
ACTATGC12150.020.2878768
TAGGTAG7450.019.4629065
TCTATAC13300.017.9885543
CTATGCA10650.017.6993689
TAGTCAG7650.017.0586649
GTCTATA10700.016.9537931
GTATAAG10800.016.7968141
GACCGCG3153.507904E-416.1109627
CCGCGCA3655.9637343E-515.8902649
ACTGCTC9200.015.7607238
TACTATG13400.015.6901527
CTAGACT5102.622146E-715.637114
GTATAGC7903.6379788E-1215.6146581
ACTCCGC5604.470712E-815.5355698
AGTACCG8450.015.4436425
GTATATA26500.015.3339061
Produced by FastQC (version 0.11.5)