FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_D05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_D05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1674179
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC3001.7096681E-824.165648
GTATAAG12550.022.5551971
CCGCGCC2053.6681772E-421.2192469
CTAGGAC4752.728484E-1019.8412633
GTATAGA13550.018.747981
TATAAGA16000.018.5778922
TATACAG15450.016.8924875
CAGCGTG4851.4809848E-716.4430929
CCTATAC9350.016.2827323
CTTAAGG6256.020855E-1016.2393113
TATATAC28950.016.0269553
GTCTAGA7402.3646862E-1115.6932911
ATAAGAG15250.015.6878593
TAGACCG8350.015.6280785
GTATTAG12200.015.4681811
CTAATAC14100.015.4248783
CTAGACC8351.0913936E-1114.7598514
TACAGTC9400.014.6536337
TCTATAC13950.014.5513533
CTATAGT9053.6379788E-1214.4192784
Produced by FastQC (version 0.11.5)