FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_D04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_D04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313708
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56080.42688329522237817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG4400.031.3066545
GTATAAG6300.026.488281
GTATAGA8650.026.0023161
GTATAAC4750.022.9120561
ACCGCGC2404.3643973E-521.1457238
CCGGCCC3809.049472E-920.9867329
GTCTAGG4952.1827873E-1120.5205651
GTCCTAC2505.9416456E-520.3153571
GTCTACA7500.020.3153571
CACCGTG3601.1768316E-720.1387847
ACCGTGT3259.835021E-720.0768188
GACCGCG2205.904926E-419.7726237
GTCTAGT5153.8198777E-1119.7236481
ACTATGC7750.019.6450588
CCCTAGG1850.00502567719.6094191
GGACGGC3401.5156347E-619.1910746
TAGACTA3802.0799962E-719.0788485
ACACAGT5707.2759576E-1219.0788486
CCACGCA3051.2755001E-519.0162939
TCTAGGA3852.3865869E-718.831072
Produced by FastQC (version 0.11.5)