FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_D03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_D03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397956
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG11000.032.9776881
GAGACAG9200.024.4290187
ATAAGAG12850.023.6961753
TATAAGA15150.022.9699862
CTAGGAG3001.106168E-519.333073
GTGTAAG6553.6379788E-1217.722361
TAAGAGA18850.017.3074594
TAATACT15600.017.1952784
GTATAGA9750.017.1145741
ACCGTGC3002.4179863E-416.9164378
AGAGACA15300.016.5847426
CCTACAC4901.6638114E-716.275293
GTATAGT10700.016.2731021
GTATATG11300.016.0510881
GTCTAGG5052.325196E-715.803171
TACTATG10850.015.3684557
TCTAGGA7102.2191671E-1015.3166932
GTATTAT20400.015.2925941
AATACAG14750.015.2370825
TATTATA25300.015.1875412
Produced by FastQC (version 0.11.5)