FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_D02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_D02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2925380
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG24950.024.4315031
ACCGCGC6000.022.9576748
CTAGGAC8450.021.449093
GTATAGA23000.020.8236831
TATACAG28050.020.4183085
GTATTAG21600.020.1576061
CTAATAC24600.019.7453693
TAATACT40550.019.4877284
TAGGTAG12800.019.257265
TAGACCG17250.018.91255
GACTAGG10050.018.755688
GACCGCG5509.822543E-1118.4540167
TATAAGA33500.018.395312
GGTAGTC15400.018.3598657
TATACCG14750.018.1859195
CCTAATA25650.018.0894582
ATAAGAG27200.017.8579443
ACTATGC23050.017.6133798
TAGACAG18600.017.5398225
GTGTAAG12050.017.464351
Produced by FastQC (version 0.11.5)