FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_C11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_C11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691620
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGG1855.8130718E-627.4322288
ACCGTGC1701.03089435E-425.5880458
CTAGGCT1751.2552418E-424.8569583
GTATAGA4950.023.4596081
CTAATAC5600.023.3033963
TATACAG6900.023.1157685
CCCTGGC1600.002174570522.6560829
GTCTAGG2252.6280408E-522.5798741
ACTATGC5850.022.3075268
TATACGG2603.2020034E-622.3075245
ACAGCGT2653.7773716E-621.886638
CTTACAC3055.3356416E-721.3932823
TATCGCG1700.003091974921.323377
TAATACT8550.021.1986714
GCCTATG2404.360634E-521.1456767
TACACCG2404.360634E-521.1456765
GTCTTAG3457.4111085E-821.037151
CTATGCA5650.020.530829
TATACCG3208.4635394E-720.3904745
CATGCAC2155.054222E-420.2324073
Produced by FastQC (version 0.11.5)