FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_C10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_C10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168116
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGT400.006010533354.4471631
CTGACCT805.850743E-754.366194
GGAGTGT400.00604604254.366198
TCCCATC400.00604604254.366192
AGACCGC450.00963146248.32556
AGACCAC450.00963146248.32556
ACGACGC450.00963146248.32558
ACTAGAC604.9372157E-448.3254973
CAGCACA657.324957E-444.6081544
TCTATGC700.00105497341.421862
CCTTACA750.001481053438.66042
CTATAGT750.001481053438.66044
TGCTCTC951.0262242E-438.1517145
TAGACCG1001.3878536E-436.2441255
GACCGTC800.002033424336.2441257
AGACCGT1051.8489406E-434.5182156
AAGGTTC850.002737705834.1121186
GGACAGT850.002737705834.1121186
CTAACAC900.003622590332.2170034
TATGTAC1153.154001E-431.516633
Produced by FastQC (version 0.11.5)