FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_C08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_C08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2377278
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG9950.034.245965
GTATTAG17650.027.1370891
CTATGCA15850.026.5297559
TAGTCAG10650.025.868349
TAGACCG13200.025.8141885
ACTATGC21500.023.941678
CCTAATA19700.023.553112
TATTAGG18850.023.4613512
GTATAGA18500.022.7520391
TTAGGTA18350.022.5202564
CCTACAC7950.021.8866183
TATACAG21250.021.8351215
AGACCGT14400.021.6491346
GGTAGTC13750.021.618017
TAATACT30150.021.4011574
GTCCTAG6800.021.3444581
CTAGGAC6800.021.3233593
TATACTG28850.021.1090185
GTCTAGG10600.020.5390071
CTAATAC21200.020.1767253
Produced by FastQC (version 0.11.5)