FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_C07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_C07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326728
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG10950.038.450091
TAGGTAG6450.033.7187775
TAGTCAG6200.031.570569
CCTACAC4400.028.0095713
TAGACCG9600.025.6754425
TATTAGG11300.025.6620752
GGACCGC1701.0319897E-425.5866016
TCGGTCG3301.7753337E-924.1651219
TATACCG6500.023.4215815
CACCGTG4650.023.3856037
CCTAGAC5950.023.149783
GGTAGTC9150.022.9766737
CTGTGCG3203.3900506E-822.6548029
TACACCG4505.456968E-1222.5541135
GTATAGA12250.022.518061
ATACCGT9700.022.4212466
CAGCGTG4307.2759576E-1121.9172049
GTCTAGG6300.021.892561
AGACCGT9950.021.8578996
TTAGGTA9650.021.7861714
Produced by FastQC (version 0.11.5)