FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_C06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_C06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512840
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17400.11501546759736656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAG2104.9908704E-727.6180523
ACCGCGC2401.5994865E-624.1657968
GTATAGA11500.023.9929311
GTATAAG11100.023.5492481
GTGTAAG6500.022.3415951
GTACTAG7900.022.058791
AGGACGG3055.345755E-721.3926725
GTATTAG9500.020.636581
GACTAGG4051.884655E-819.6906478
TAATACT18050.019.6807334
TATACAG13450.019.4045055
TATAAGA15750.019.3326382
GGGCGCG1900.005889355719.078899
GTATATG13100.018.845391
TAGACCG7350.018.7408225
ATAGGAC7100.018.3796223
GTCCTAA6451.8189894E-1218.0118271
GAGACAG10500.017.9517357
AGACCGT9050.017.6236746
GTATATA21650.017.439861
Produced by FastQC (version 0.11.5)