FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_C05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_C05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180426
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG17600.025.9751321
GTGTTAG13900.019.8380371
TAATACT29250.019.8287564
GTATAGA16550.019.2923241
GTATTAT32200.018.7047581
GTCCTAA9350.018.6264271
GTATATA31200.018.3739451
TATAAGA24750.018.1617552
TAGGTAG8900.017.9210745
ATAAGAG20900.017.6911163
ACCGTGC4659.168434E-817.1502748
GTATAGG12200.016.6543661
CTAATAC16850.016.34993
GGACCGT5352.5507688E-816.2614336
GTATAAC14300.016.2384221
GTATATG20250.016.1256541
GTATTAG15900.015.9735271
GTCTAGG8300.015.7370851
TATACTT30100.015.6559075
ATTATAC22950.015.4790663
Produced by FastQC (version 0.11.5)