FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_C03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_C03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587924
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGG4400.028.0094388
ACTAGGC4850.025.4122249
GTCCTAA6450.022.5031641
AGACTAG5800.022.499167
ACCGCGC2353.7129204E-521.5942578
GTCCTAC3706.5811037E-921.575671
GTATAGA12450.020.9848791
GACCGCG2455.1179042E-520.7135127
TACACCG5056.202754E-1018.662675
CCCCGGC2751.2428385E-418.454449
GACCGAC2050.00913066717.6822647
TATAGAG12800.017.5579382
TATACCG7600.017.170415
GTATAAG12350.017.0413651
GTGTATG9850.016.945911
GTATAGG8600.016.8773731
GTATTAG10800.016.7992361
ACGGCGC2600.001813200116.7296188
TATACTG15700.016.6235835
TATACAG14050.016.5118395
Produced by FastQC (version 0.11.5)