FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_C02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_C02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429430
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG18100.022.0522821
TACACCG9200.021.2764035
ACCGCGC3851.0535587E-820.7135358
GTATAGA19350.020.2526661
ACCGTGC5851.2732926E-1118.5890718
GTATTAG15800.018.3726731
ACTATGC17850.018.276658
CTATGCA14850.018.063329
TATAAGA23850.017.6311152
TATACCG9900.017.575125
TAGACCG12400.017.5396885
CTATACT17400.017.4993674
GACCGAC3403.2498858E-517.0582077
ACTGTGC9850.016.9283228
TATACAG18450.016.8964085
GTTATAC13400.016.771783
GTATTAA22600.016.6979941
TACTATG19400.016.4427037
TAATACT30200.016.0838554
CCTAATA19450.016.0283572
Produced by FastQC (version 0.11.5)