FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_C01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_C01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3988531
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60020.1504814679890917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG18800.025.8365975
TAGTCAG16750.025.1042889
GTATTAG32200.022.5396581
GGTAGTC20800.020.9122527
ACCGTGC8450.020.5905278
GTATAGA32550.018.5067541
CCTAATA33200.017.6878972
CTAATAC34700.017.1320043
TATTAGG32600.016.6791132
CTATGCA25100.016.4640189
CCGTGCG4851.4824218E-716.4431749
TATACAG37950.016.4287685
TAGACCG22100.016.4019745
GTCCTAA16050.015.8269121
ACTATGC34150.015.7091948
CCTATAC24000.015.707823
AGACCGT25850.015.4248346
GTGTAAG18450.015.3416271
TTAGGTA31800.015.2746584
GACTAGG14400.015.1032938
Produced by FastQC (version 0.11.5)