FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_B11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_B11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5039488
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG39900.017.4613671
TAGGTAG20250.016.1107125
TAATACT72150.015.2733564
GTATATA91150.015.2074581
TATACAG50800.015.1275795
ACCGCGC8309.094947E-1214.849038
CTAATAC41750.014.7601143
GTATAGA41500.014.1650081
CTAGGAC13200.013.730723
GTATATG54550.013.7032131
TATACTG55950.013.4759855
TATTATA113450.013.1004212
CTCCTAG11650.013.0819731
TATACCG26100.013.0552325
ATAATAC79750.012.9996783
ACCGTGC10603.6379788E-1212.9949628
ATTATAC65050.012.8167463
TATTAGG44350.012.7507832
TATAATA113800.012.6778812
TATACGG28150.012.6195815
Produced by FastQC (version 0.11.5)