FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_B10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_B10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4294056
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA26250.021.5633131
TACACCG10150.020.713195
ACTATGC24000.020.2385148
CTAATAC27250.018.888913
AGACCGT20800.018.8211216
TAGACCG16450.018.509665
GTACTAG15700.018.4888781
TACTATG25650.018.371357
CTATGCA20700.018.2124469
CTAGGGT7650.018.0055834
GTCCTAA14950.017.960181
GTCCTAG8500.017.928771
TAGGACA15400.017.8886644
GTATTAG23350.017.7148781
CTAGACT11350.017.2457854
CCTAATA24750.016.9892
TCTAGAC19700.016.928043
TATACAG24900.016.886665
CCGTGCG3453.6834943E-516.811499
TAAGGTG23350.016.4552355
Produced by FastQC (version 0.11.5)