FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_B09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_B09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2081070
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG7000.022.784865
TCCTTGC11300.021.8133418
CCCCTAT6100.021.4168241
CCTGTAC7600.019.0782323
TGGACCG4954.7475623E-1019.0396925
TCCTAGG3051.2761753E-519.0161382
ACTCCGC7150.018.2510648
TCCTAAG6401.8189894E-1218.1247582
GTATTAG13050.017.7972091
GTCCTAG4908.5037755E-917.7745081
CCCTTAT13350.017.397271
CTCCGCA8350.017.3650369
ATCCTTG17150.017.3317137
TATACAG13550.017.121135
TACTCGG3854.9703885E-616.9474167
CCTATAC9500.016.7888453
GACCGCG3052.7443582E-416.638727
GTATTAT21100.016.5109181
TTAGGTA11450.016.4622654
CCTTGCA11150.016.2553869
Produced by FastQC (version 0.11.5)