FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_B08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_B08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6824632
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA59850.022.6765021
GTATTAG52600.022.0766331
TAGGTAG31350.021.9693225
TCTATAC58200.018.685253
CTAATAC60750.018.4976273
TAGTCAG28800.017.621239
TATACAG66900.017.4473865
CTATACT57100.017.0137214
GTACTAG35650.016.8972931
TATTAGG58800.016.6453692
TATACTG80900.016.3995975
ACCGTGC17400.016.2497238
TATACGG38900.016.2143765
TAGACCG34500.016.1808835
GGTAGTC33750.015.6812157
ACTATGC56900.015.6719668
TAATACT89050.015.5499884
TAGACAG42800.015.2450695
CTATGCA46550.014.951419
GTCTAGG29650.014.9315111
Produced by FastQC (version 0.11.5)