FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_B07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_B07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5931257
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG27200.023.9881085
GTATAGA44900.023.9206871
CCGTGCG6550.023.2440269
GTATTAG44100.023.2027151
TACACCG17100.022.469955
CTAATAC47800.021.9918863
CCTAGAC23800.021.0181523
TATACTG62800.020.7795075
CTATGCA31900.020.4543259
CTATACT43050.020.3767364
AGACCGT34950.020.1208786
TAGGACA25950.020.1148834
TATACAG47500.019.841395
TCTATAC46800.019.8283463
ACTATGC42950.019.5800388
GTCCTAA25250.019.5436551
GTCTTAG25650.019.238881
ACAGTGC25700.019.1820288
CTAGACT17950.018.9826034
CTAGACA26450.018.9123634
Produced by FastQC (version 0.11.5)