FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_B06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_B06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925036
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9920.10723906961458797No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG6800.033.0923161
ACCGCGC1358.0826745E-426.8516128
TAGGTAG4900.026.6324145
ACCGTGC2507.830204E-826.0997688
TCGGTCG2051.2902174E-524.7558789
TATACAG9100.023.9008855
TAGACCG4950.023.4341345
GTGTAAG5300.023.2834571
ATTAGGT7800.022.3074933
TATACGG5250.022.095045
CTGCGCA2003.1029823E-421.7498059
TAGTCAG4358.367351E-1121.6664719
AGTGTGC2404.3627275E-521.1456458
GTCCAGG2755.1545285E-621.1169741
TACAGCG3809.042196E-920.9866547
TATTAGG8000.020.8446882
AGTCCGT2104.3140567E-420.71418
AGACCGT5251.8189894E-1220.71416
TACACCG3508.735151E-820.71415
GGTAGTC5301.8189894E-1220.5186847
Produced by FastQC (version 0.11.5)