FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_B05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_B05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218252
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG9350.024.8345931
TAGGTAG5900.022.117145
CCTAATA8050.019.8123192
TTAGGTA8000.019.0299574
TATTAGG8500.018.7634322
ATAAGAG10850.018.7084063
GTCTTAT7450.018.5061451
ACTATGC9800.018.493648
GTATTAG8250.018.4707281
TACTATG10750.018.2080657
GTATATA16750.018.1950451
TATAAGA12750.018.1948412
CTATACA8750.017.3988174
TAATACT13400.017.3122564
CTGTACT7150.017.2365914
TATACAG10150.017.1416935
TATAGCG4256.7349356E-717.0576655
GTATAAC9000.016.9315011
CTATACT8600.016.859324
GAGACAG8800.016.4761547
Produced by FastQC (version 0.11.5)