FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_B04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_B04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891819
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG14700.028.140311
TATAAGA17700.024.9857032
CCGTGCG1801.5210565E-424.1664989
ATAAGAG15550.022.84553
GTATAGA14800.022.5562821
TAGGTAG6500.021.1921625
GTATTAG12600.020.7349661
CTCCGTG3157.289327E-720.7141427
GAGACAG14300.020.2795817
CTAATAC14650.019.7950863
GTCTAGG8200.018.585611
GTCCTAA8550.017.8247971
CTAGGAC6202.910383E-1117.5402013
TAAGAGA23250.017.4622444
GTAGGAC5001.10649125E-817.399883
ACCCTGC8350.017.365158
TATACGG9350.017.0587065
GTATAAT18450.016.9138891
TAATACT21400.016.6003514
TATTATA30600.016.5848522
Produced by FastQC (version 0.11.5)