FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_B03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_B03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991980
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG11450.019.6453271
GTATAGA15600.019.5356431
CTAGACT6350.019.409194
GTATAAG15650.019.009581
GTACCGC5005.4387783E-1018.8497476
TAGGTAG7700.018.8309175
GTATTAG12800.018.7071231
TAGTCAG7400.018.6146169
TATTATA33650.018.5287272
GTATATA30450.018.3487471
GTCCTAA9200.018.140241
TAATACT25300.018.053124
TATACAG17350.017.968065
ACCACGC4104.6247442E-717.682258
TCGGTCG4557.1520844E-817.5272399
GTGTATA16300.016.9160521
CCTAAGG7850.016.6239813
TCCTAAG10050.016.5918182
GTATTAT27850.016.1536081
ATAATAC29450.016.0014843
Produced by FastQC (version 0.11.5)