FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_B02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_B02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1844591
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG13800.027.3512481
ACCGCGC3503.6379788E-1226.9276318
AGACCGC5000.026.0997966
GTCCTAG4850.023.9458981
GGACCGT4103.8198777E-1122.9876236
ACCGTGC3955.9117156E-1022.0245468
CTCCTAG3757.668859E-921.2918951
TATAAGA20850.020.8642772
CTAATAC14250.020.8594863
TAGGTAG7700.020.7141235
CCGCTCG2155.058629E-420.2323999
GTATAGA14000.019.7019181
CCGCGCA3701.5717524E-719.594449
GTATTAG13800.019.4614661
ATAAGAG17900.018.631143
GACCGTC7900.018.3542867
GACCGCG3953.126479E-718.3542867
GACCGCT4354.276626E-818.333197
ACAGCGT8450.018.0171228
GCCGCGC2450.001217739517.7544828
Produced by FastQC (version 0.11.5)