FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_A11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_A11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4006905
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG31500.019.5847021
TAGGTAG18300.016.6386765
TCTATAC32200.016.4356753
GTATAGA34600.016.361651
CTAATAC35950.016.334533
TATACCG19650.016.2334445
TATACAG38650.015.9437315
CTAGGAC11550.015.6920093
CTATACA31800.015.5025224
TATACGG22900.014.8793145
GTACTAG21100.014.79091
ACTGTGC17650.014.7867568
TATACTG47200.014.7451715
GTGTAAG17800.014.6788171
TAGACAG22650.014.4033955
CCTATAC25800.014.3308193
TATTAGG35300.014.1708182
GTATATA72150.014.0831641
ATACCGT27450.013.9976176
AGGGCCG5751.020222E-613.8690085
Produced by FastQC (version 0.11.5)