FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_A10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_A10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298378
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG3750.028.9988489
TAGGTAG14350.027.2811475
GTATTAG25150.022.7969911
TAGACCG18350.020.9392225
GTCCTAG8700.020.8549521
TAGTCAG15000.020.7825099
TATACAG33050.020.6194535
TACACCG12400.019.8782425
CAGCGTG11350.019.1622339
GTATATA55900.018.955251
TTAGGTA25700.018.9000264
GGTAGTC18050.018.8773657
GTATAGA28300.018.720821
TATTAGG26750.018.7001912
TATACCG16800.018.555815
AGGACCG7300.017.8760015
TCTATAC28250.017.7072623
CTAGACT10250.017.6822224
TATACTG39300.017.1558075
ACAGCGT17450.017.0337078
Produced by FastQC (version 0.11.5)