FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_A09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_A09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751074
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG12700.025.1167095
GTATTAG23900.022.7741991
TACACCG10250.019.0964975
TATACAG30150.018.9956255
GGTAGTC13250.018.6027367
TAGTCAG12950.018.4745459
TATACGG16350.018.1794135
TATTAGG25500.017.627112
ATACAGT26350.016.7827456
GTATATA57150.016.7624471
CCTACAC7850.016.6232783
TCTATAC25050.015.9172753
GACTAGG8250.015.8172998
GTGTAAG14700.015.7983741
ACAGTCC11500.015.7599918
ACTATGC21800.015.6298628
TTAGGTA20100.015.5090854
CTAGTAC17900.015.3902033
ACTGTGC12250.015.3868968
GTCTACA18400.015.3824861
Produced by FastQC (version 0.11.5)