FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_A08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_A08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4769472
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG19300.020.6599775
CTAATAC40050.019.7307623
GTATTAG37000.018.6314721
GTATAGA40200.018.5924431
CCTAATA42850.018.1034742
CTATGCA30550.018.0356339
TATACTG56850.017.4708715
CCTATAC28650.017.4600053
ACTATGC39200.017.0147748
TAATACT62100.016.8108924
GTATATA79000.016.8092861
GTATAGG32950.016.5169981
TAGACCG25350.016.3012665
TATACAG45450.016.2701195
CCCCTAG6459.313226E-1015.7504741
TATACGG27200.015.7256115
ACCGTGC11200.015.5352298
GTACTAG25750.015.4992521
CTAGGAC15050.015.414643
TAGACAG30050.015.1991925
Produced by FastQC (version 0.11.5)