FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_A07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_A07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4825952
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG20050.024.5875745
GTATAGA26200.024.0997091
ACCGTGC9400.023.137168
GTATTAG27000.022.3104421
TAGGTAG14050.022.1877525
TACACCG13350.021.7220045
ACCGCGC8000.020.8427268
CCGTGCG4301.7043931E-920.23249
AGACCGT24950.020.049326
CTATACT28400.019.6559284
TATACCG18350.019.3589215
TCTAGAC20600.019.3560453
TATACGG17450.019.1110065
CTAGGAC11400.019.0782073
CCTACAC12300.018.8610573
GTCCTAA19000.018.7170031
GTACTAG19400.018.705191
CCTAGGG7000.018.6421343
CCGCGCG6301.8189894E-1218.4125589
CTAGACT12800.018.1242984
Produced by FastQC (version 0.11.5)