FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_A05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_A05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1677962
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG12450.030.8955381
TATACGG6500.022.3062615
TAAGAGA18950.022.188554
CAGCGTG4501.3278623E-1020.9443519
AGGACCG4501.3278623E-1020.9431025
ATAAGAG14650.020.7836513
TATACTG16050.020.7774835
GTGTATA12300.020.6515271
TATAAGA16650.020.4641532
CCGGCCC2505.9777994E-520.2999089
GTATAGA12900.019.690991
GTATTAG11900.019.5160661
GAGACAG12100.019.1723257
TCTAGAC7600.019.0777233
TATACAG12050.018.6502575
TATTAGG12250.018.3457642
TCTATAC12100.017.9740543
CTATACA10200.017.7684674
CGTGGGC4557.154267E-817.526358
CCTAATA10350.017.5109542
Produced by FastQC (version 0.11.5)