FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_A04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_A04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774596
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC2651.3778663E-724.622118
GTATAAG11650.023.0486351
GTATAGA11850.022.6596281
TATACAG11450.022.1610955
CTAATAC11400.021.6223413
CTAGGAC4851.6370905E-1120.9273823
TAATACT16450.020.713844
CGGGGCT3508.747702E-820.7138394
AGACCGT10000.020.2995626
ACTGTGC6850.020.1090568
ATACTGG11250.019.9773466
TAGACAG9500.019.8416775
CTAGTAC8500.019.6172263
TAGACCG9250.019.5941725
GTCTACA12600.019.0069641
GTGCTAG7650.018.9730851
GTCTAGG6201.8189894E-1218.7282711
TATACTG17100.018.654575
CTAGACT5158.058123E-1018.3005774
GTATTAG10800.018.1430131
Produced by FastQC (version 0.11.5)