FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_A03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_A03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116788
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG7450.032.1389851
ACCGTGT2654.5638444E-927.3564058
TCTATAC9650.027.0445693
TAGGTAG4350.024.9980935
TGGACCG2751.970493E-723.7254645
GACCGTG2451.9136842E-623.6716637
CAGCGTG4154.3655746E-1122.7111459
TATTAGG7900.022.0236382
TATACAG10250.021.9251565
CCGTGTA2003.1030728E-421.750299
TACAGCG5350.021.680597
ACTGCGC2353.7118625E-521.5940978
TAGACAG6500.021.1906915
TATACCG4451.1459633E-1021.178165
ATACAGC8650.020.9521586
CACTGCG2806.095339E-620.7127087
GTATAGA8200.020.3511281
GGACCGT2556.9624955E-519.9004426
TAGCACT7850.019.3934274
TAGACTA4152.492925E-819.2154035
Produced by FastQC (version 0.11.5)